American Society of Microbiology ABMM : ABMM

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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is preparing a Gram stain of a mixed bacterial culture containing both Gram-positive and Gram-negative organisms. The correct order of reagents used in the Gram staining procedure is:

A) Crystal violet, iodine, decolorizer, safranin
B) Safranin, crystal violet, decolorizer, iodine
C) Iodine, crystal violet, decolorizer, safranin
D) Decolorizer, crystal violet, iodine, safranin

2. Transmissible spongiform encephalopathies (TSEs), such as Creutzfeldt-Jakob disease (CJD) in humans and Bovine Spongiform Encephalopathy (BSE) in cattle, are caused by infectious agents composed primarily of:

A) Slow-growing bacteria lacking a cell wall
B) Viroids (small, circular RNA molecules)
C) Aberrantly folded host-encoded prion proteins (PrP<sup>Sc</sup>)
D) Defective viral particles requiring a helper virus

3. A 55-year-old male with a history of poorly controlled diabetes mellitus develops a necrotizing fasciitis of his left lower extremity following a minor abrasion sustained while gardening. Initialcultures of the wound reveal a polymicrobial flora, including Gram-positive cocci in chains and Gram-negative bacilli. Over the next 48 hours, despite broad-spectrum antibiotic therapy, the patient's condition deteriorates, and new bullae with hemorrhagic fluid appear. Gram stain of this fluid now predominantly shows large, Gram-positive bacilli with subterminal spores. Which of the following virulence factors is MOST likely contributing to the rapid tissue destruction observed in this patient?

A) Protein A
B) Lipopolysaccharide (LPS)
C) Exotoxin B (SpeB)
D) Lecithinase (alpha-toxin)

4. A patient develops a localized skin infection after being bitten by a tick. The infection is characterized by a slowly expanding erythematous rash with central clearing (erythema migrans). The MOST likely causative agent is:

A) Ehrlichia chaffeensis
B) Borrelia burgdorferi
C) Rickettsia rickettsii
D) Anaplasma phagocytophilum

5. A patient with a history of intravenous drug use develops a fever and new heart murmur. Blood cultures are positive for Gram-positive cocci that are catalase-positive and coagulase-positive. The MOST likely complication of this infection is:

A) Embolic stroke
B) Septic arthritis
C) Glomerulonephritis
D) Reactive arthritis

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